Typing Staphylococcus aureus

Overview of S. aureus

Staphylococcus aureus is a well- known human pathogen and a growing concern in veterinary medicine. Indeed, this bacteria is one of the main etiological agents of ruminants mastitis, a major cause of economic loss in the dairy industry. It is moreover a major cause of food-borne disease by its ability to produce heat-stable enterotoxins. In order to implement control measures targeted at reservoirs and transmission routes, Ceeram has been developing a powerful typing method.

What is Bacterial Typing?

A bacterial typing method is a phenotypic or genetic analysis of bacterial isolates performed to generate isolate-specific fingerprints for assessing epidemiological relatedness. This analysis is based on polymorphic phenotypic or genetic biomarkers to differentiate each individual in the entire specie.

Why typing S. aureus?

Reveal outbreak cases to initiate community containment measures.

Check if human handling is the source of a contamination to implement hygiene measures.

Check if breeding device or milking machine are the source of a contamination.

Evaluate the impact of treatment to guarantee its efficiency.

Determine the source of food contamination (human or animal) as a microbial source tracking tool.

Emphasize the emergence of virulent strains/clones (CC398, CC9,...)

Study S. aureus epidemiology to know its evolution and evaluate emergence conditions.

Download our leaflets for both S. aureus in Food and S. aureus in Health sectors.

Genotyping kits for S. aureus?

Efficient amplification of the 16 markers in 2 multiplex PCRs.
Required equipment: ABI sequencer to analyze the amplicons.
The typing data is managed by GeneMapper (defaultly linked with ABI sequencers) to obtain the numeric code.
Ceeram provides specific and adapted training sessions to help the implementation of bacterial typing in your lab.

mardi 30 août 2011