The methods used for the detection of foodborne viral infections consist in:
1- an extraction process from several type of matrices :
FOOD: drinking water, process water, pre-cooked and ready-to-eat meals, shellfish, fruits and vegetables, aromatics, swaps
ENVIRONMENT: raw water, sewage plant water, drinking water, surface water, sludge, swaps, air and surface samples
HEALTH: biological samples (blood, urine, stools, tissues), swaps
2- the amplification of the viral extraction control (with real time QRT-PCR):
The French laboratory also developed a GMO non pathogenic extraction control to ensure that the extraction yield is optimum.
3- and the use of the ceeram tool kit for the molecular detection for quantitative one step real time RT-PCR (Retro Transcription, Polymerase Chain Reaction Real time).
These test kits include :
- primers and Taqman probes designed for conserved region of targeted viral genome
- optimization of each test (inclusivity/exclusivity tests, sensitivity, robustness of the assays),
- construction of internal positive control for amplification control,
- construction of specific standard for quantification.
Thanks to these kits, ceeram can test several pathogens:
- VIRUS: Norovirus (GI and GII), Hepatitis A virus, Rotavirus, Adenovirus, Hepatitis E Virus, Enterovirus, Astrovirus, AichiVirus, Circovirus, etc.
- BACTERIA: Brachyspira (pilosicoli, intermedia, hyodysenteriae), etc.
- PARASITES: Cryptosporidium spp., Giardia spp., etc.
In order to perform with the tests, and get the best results, ceeram provides training (approved training centre), support and assistance, as well as R&D for specific development (Approved Research Centre).
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| Those kits are:
- easy to perform,
- give reliable results,
- are specific to genotypes detected,
- have an excellent sensitivity,
- are adaptive to most thermocyclers,
- give results in less than 2:30 hours.
ceeramTools Kits can be used in Food, Health, Environment sectors for testing any type of samples.
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