jeudi 2 février 2012

Hospital clear of virus

Hospital clear of virus - News - Weston Mercury

The norovirus had caused disruption to several hospital wards since the first outbreak in mid-December.


Genogroup I and II noroviruses are non enveloped and single strand RNA viruses. These human enteric viruses are responsible for most of viral gastroenteritis outbreaks and pathogenic for all age groups of the population. Noroviruses are very infectious; one to ten viral particles are sufficient to get sick. Their mode of transmission is the feco-oral route. After excretion in human stool, due to their structural properties, noroviruses can resist to different physical and chemical treatment.
Adsorbed on particles, noroviruses can survive in different kind of environment for a long period. Food samples can be contaminated via food handlers or by the environment. Due to their structural properties, noroviruses can survive to different food process. The consumption of contaminated food can lead to human outbreaks.

There is no cell culture system available for the multiplication of human noroviruses. The detection by ELISA is not recommended due to norovirus genetic diversity and its lack of sensitivity. Molecular techniques (real time RT-PCR) are the method of choice for NoV detection after extraction and viral RNA purification from the sample.

ceeramTools™ novovirus GII assay is based on real-time PCR. Real-time PCR is a proven method for pathogen detection and has been applied successfully to a wide range of foodborne pathogens. The method tests for food pathogens by amplifying and detecting a RNA target sequence that is specific to Norovirus. Detection is quick, simple, and with outstanding sensitivity and specificity. Shelf-Life : 360 days.

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