dimanche 15 avril 2012

Harmonization of Methods for the Extraction and Detection of Human Enteric Viruses from Foods

Speakers will present on their respective efforts to develop harmonized and/or standardized methods for detection of enteric viruses in food products. The European perspective will discuss the efforts of the European Committee for Standardisation TAG4 group (comprising scientists from 13 European countries) to develop a standard method for detection of viruses (including norovirus) in a number of food matrices (bivalve shellfish, soft fruit, salad vegetables, bottled water, food surfaces). The method will be published this year as an ISO Technical Specification – in addition the European Union is currently funding TAG4 to conduct a validation project which will enable the method to be re-published as a validated International Standard within the next few years. The U.S. perspective will focus on the recent development of a rapid and sensitive method for the extraction and detection of hepatitis A virus and norovirus from green onions and bivalve shellfish. This presentation will discuss progress made on validation of these methods and how these are being incorporated into FDA’s regulatory mission. The Canadian perspective will focus on the successful collaboration of several federal and provincial laboratories that resulted in the development and validation of a norovirus detection method in oysters. By 2006, the method designated as OPFLP-01 was published in the Health Canada (HC) Compendium of Methods following validation and approval by the Microbiology Methods Committee (MMC). The method has performed well in the EU Ring Trials and has been used to aid in the investigations of various foodborne illnesses.


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