P. aeruginosa is an ubiquitous environmental bacterium and a human pathogen. Due to its large occurrence in hospital water systems and its faculty to persist on medical devices, P. aeruginosa is a leading cause of hospital-acquired pneumonia. To track the primary source of infection and the spread of P. aeruginosa in the health care facility, Ceeram has been developing a powerful typing method.
A bacterial typing method is a phenotypic or genetic analysis of bacterial isolates performed to generate isolate-specific fingerprints for assessing epidemiological relatedness. This analysis is based on polymorphic phenotypic or genetic biomarkers to differentiate each individual in the entire specie.
WHAT IS BACTERIAL TYPING ?
MLVA, A POWERFUL TYPING METHODMulti Locus VNTR Analysis (MLVA) is a PCR based typing method that relies on the inherent variability found in many regions of repetitive DNA called VNTR (Variable Number Tandem Repeat) which represent sources of genetic polymorphism. The MLVA assay for P. aeruginosa examines 16 loci. Thus, each isolate is defined by a 16-digit numeric code corresponding to the number of repeats at each VNTR.
WHY TYPING P. aeruginosa ?
- Identify and trace outbreaks in a real time from a single or a multiple source.
- Check if a medical device is the source of a contamination to implement hygiene measures.
- Distinguish recurrence and reinfection in a chronic patient to determine the source of infection.
- Check if the hospital water network is the source of contamination to characterize the outbreak (nosocomial or community-acquired).
- Show evidence of cross-infections between patients to control the outbreak.
- Emphasize the emergence of virulent strains/clones (Lesb, PAO1, PA14,...)
- Study P. aeruginosa epidemiology to know its evolution and evaluate emergence conditions.