lundi 23 janvier 2012

MRSA in Conventional and Alternative Retail Pork Products

PLoS ONE: MRSA in Conventional and Alternative Retail Pork Products

Genotyping Staphylococcus aureus

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Overview of S. aureus

Staphylococcus aureus is a virulent pathogen that can cause a wide range of diseases from mild skin infections to life-threatening sepsis. S. aureus is currently the most common cause of nosocomial infections and a major foodborne pathogen. In order to implement control measures targeted at reservoirs and transmission routes, Ceeram has been developing a powerful typing method.

What is Bacterial Typing?

A bacterial typing method is a phenotypic or genetic analysis of bacterial isolates performed to generate isolate-specific fingerprints for assessing epidemiological relatedness. This analysis is based on polymorphic phenotypic or genetic biomarkers to differentiate each individual in the entire specie.

Why typing L. pneumophila

  • Identify and trace outbreaks in a real time from a single or a multiple source.
  • Check if a medical device is the source of a contamination to implement hygiene measures.
  • Distinguish recurrence and reinfection in a chronic patient to determine the source of infection.
  • Show evidence of cross-infections between patients to control the outbreak.
  • Determine the source of food contamination (human or animal) as a microbial source tracking tool.
  • Emphasize the emergence of virulent strains/clones (CC8, CC45,…)
  • Study S. aureus epidemiology to evaluate emergence conditions and seasonal influences on contaminations.

MLVA, a powerful typing method

Multi Locus VNTR Analysis (MLVA) is a PCR based typing method that relies on the inherent variability found in many regions of repetitive DNA called VNTR (Variable Number Tandem Repeat) which represent sources of genetic polymorphism. The MLVA assay for S. aureus examines 16 loci. Thus, each isolate is defined by a 16-digit numeric code corresponding to the number of repeats at each VNTR.

Genotyping Kit

Efficient amplification of the 16 markers in 2 multiplex PCRs. Required equipment: ABI sequencer to analyze the amplicons. The typing data is managed by GeneMapper (defaultly linked with ABI sequencers) to obtain the numeric code. Ceeram provides specific and adapted training sessions to help the implementation of bacterial typing in your lab.

Ceeram Solution : Service

Our Expertise Department has an automatized and standardized genotyping platform at disposal. The procedure involves three steps:

  • Samples receipt (DNA or colonies on plates)
  • MLVA genotyping and
  • Analysis report (isolates relatedness).

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